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TRAC 9: Polymerase Chain Reaction and Molecular Hybridization Technology
This lecture and laboratory course will focus on two important technologies which share strong interfaces with basic molecular biology research, genetics, molecular pathology (including cancer and genetic diseases), and identification of viral, bacterial and protozoan pathogens, namely the polymerase chain reaction and molecular hybridization technology.
Topics: Molecular Hybridization Topics include Probe Design, Optimization, and Application at the Chemical and Cytological Levels; Principles of Hybridization; Base Structure, Modification, Stability and Specificity; Tm and Cot1/2; Preparation of Chemical and Cellular Samples; Hybridization Formats: Target Abundance vs. Probe Abundance, Spots/ Blots, Southern and Northern Analysis, Soluble Targets, in situ Hybridization; Radioactive, Biotinylated, DNP, Chemoluminescent and Oligonucleotide Probe Synthesis, Purification and Use; Label and Signal Amplification and Hybridization Accelerators.
The Polymerase Chain Reaction Topics include the PCR and it's Optimization, Thermostable DNA Polymerases; Amplification of Genomic DNA and cDNA; use for RNA Amplification and mRNA Quantitation; Probes and Primers; Synthesis and Labeling; Research Applications; Recombinant PCR, Cloning, Sequencing, DNA Polymorphisms, Screening of Libraries; Clinical and Forensic Applications; Sample Preparation, Detection of Viruses and Bacteria, Oncogenes and Cancer, Familial Relationships; in situ PCR and PRINS; Contamination Control.
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