TRAC 8: Immunochemistry and Monoclonal Antibody Production

The objective of this lecture and laboratory course is to provide investigators with information on chemical approaches to the isolation, purification and characterization of antibodies and antigens. Special emphasis will be given to monoclonal antibody formation, assay, and characterization.

Topics: The Immune Response; Immunoglobulin Structure and Diversity; Immunogenetics; Antigenic Determinants; Idiotypic Network; Application of Monoclonal Antibodies; Cell Fusion; Myeloma Cell Lines; Screening for Monoclonal Antibodies (ELISA, Immunofluorescence, RIA); Cloning; Purification and Characterization Approaches; Human Monoclonal Antibody Formation and use; Crystal Structure; Purification of Antibodies from Serum, Ascites, and Culture Supernatant; Analysis of Antibody-Antigen Reactions by Immunoprecipitation and Equilibrium Dialysis; Affinity Determinations; Production of Idiotypic Antibodies, Antibody Fragments, and Novel Antibodies; Peptide Carriers and Antigen Synthesis; Radioreceptor and Crosslinking Assays for Cell Surface Receptors; Immunocytochemistry; Recombinant DNA Approaches.

Lecture:
9:00 - 4:00 p.m.
Monday
8:30 - 11:30 a.m.
Tuesday-Friday

Laboratory:
12:30 - 5:30 p.m.
Tuesday-Friday