TRAC CRM49: Making iPSC From Blood
Co-Sponsored by the NIH Center for Regenerative Medicine (CRM)

Recent advances in generating iPSCs now allow for their derivation from blood. This recent advance enables basic and clinical researchers to reprogram a blood cell into an iPSC and then further differentiate into any cell type. This capability allows researchers to develop "disease in a dish" paradigms to investigate disease and therapy mechanisms.

In this one week workshop, participants will learn how to generate iPSC from blood samples using a non-integrating approach. Due to the length of this procedure (iPSC generation ~3-4 weeks etc.) starting material (CD34+ cells or mononuclear cells) will be provided for each investigator and only critical stages will actually be performed during the laboratory portion of the workshop.

In addition to learning how to culture cells and reprogram blood cells into iPSCs, we will also present some of the latest methodologies for directing differentiation of these iPSCs into different lineages (e.g. hepatocytes or cardiomyocytes). Therefore, this course will package together the essential methodology to take a CD34+ cell isolated from blood, reprogram this cell, and then direct differentiation into multiple different lineages.

Topics: Introduction to Mouse and Human Induced Pluripotent Stem Cells; Cellular Reprogramming Methodologies, Reprogramming from Blood; Techniques Used for Blood Cell Purification and Culture; CD34+/WBC Purification and Culture; Transfection of Human CD34+ Cells With Lenti/Sendai Viruses; Human Pluripotent Stem Cell: Growth Conditions, Handling Methods, Expansion and Cryopreservation; Differentiation of IPS to Major Lineages; Preparation of Cells From Embryoid Bodies To Study Differentiation; Differentiating IPSC to Blood Cells, Endothelial Cells, Neural Cells, Hepatocyte Lineage; Stem Cell Marker Analysis

Benefits for Early Registering:

• First 15 registered participants will receive an iPSC line generated by NIH CRM, a $20,000 value (must register before May 17, 2013)
  
  
• Register by June 28, 2013 to take advantage of the early registration discount ($995). The registration fee will increase to $1,095 on June 29, 2013
  
  
• Class size will be limited to 24 (waiting list anticipated)
  
  
  

Related Bio-Trac Course:
Bio-Trac 18: Stem Cells

Bio-Trac 43: iPSC I

Bio-Trac CRM 47: iPSC II: Induced Human Pluripotent Stem Cells (hiPSC); Differentiation to Neural Lineages

Bio-Trac CRM 48: Using TALENs for Gene Engineering

9:00 - 5:00 p.m.