Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 10
Room 1N241 -
MSC 1115
Bethesda, MD
20892-1115

301-496-8290

 

TRAC CRM48: Using TALENs for Gene Engineering
Co-Sponsored by the NIH Center for Regenerative Medicine (CRM)

Gene engineering provides the ability to manipulate gene expression in a desired cell type. In order to realize the full potential of stem cells, the development of tools to modify targeted genes is paramount.

Transcription Activator-Effector Like Nucleases (TALENs) are artificial restriction enzymes that allow for efficient, programmable, and specific DNA cleavage. This recent technology offers precise engineering of your gene of interest and has been shown recently to specifically mediate site-specific genome modification in mammalian cells including iPSCs.

During this 3-day workshop, participants will learn the principles and methods of TALENs and given the base knowledge to create their own TALENs.


Topics: Lecture: Introduction of Designer Nucleases, From ZFN to TALEN (A Review of Moving From ZFN to TALEN, Various TALEN Platforms and their Applications Available Today); Gene Deletion in Mouse B cells and ES cells Using TALENs (Protocols of Efficient Screening of Targeted Cells, Discussion of Gene Targeting Strategies); DNA Repair Mechanisms (Scientific Background Behind the Technology, Case-Study Discussions Along With Time to Discuss Circumventing Technical Issues and Troubleshooting); TALEN-Mediated Knock-in Reporter in Human iPS Cells; Using TALEN in Human, Mouse and Zebrafish Research

Laboratory: Introduction of TALEN Design Website and Software; Set up Golden Gate TALEN ligation; Ligation Results, Pick Colony; Computer Design of Your Own TALEN; Miniprep, Digestion; Set up Final Golden Gate TALEN Ligation

Class Limit of 24

Benefits for Early Registering:

  • Register by June 1, 2013 to take advantage of the early registration discount ($895). The registration fee will increase to $995 on June 2, 2013

  • First 15 Participants Registered by June 1, 2013 will receive at no additional charge from the NIH Center of Regenerative Medicine (CRM) a Safe Harbor TALEN ($200 commercial design), a donor vector ($1,000, commercial design), and a cell line ($200 - $500, commercial order) Note: MTA must be signed in advance.

  • Should you wish to use the course to start your own project, participants can submit a target site and we will help you design it during the course.

  • One Submitted TALENs will be used to practice hands-on TALEN Assembly Experiment.

Related Bio-Trac Course:
Bio-Trac 18: Stem Cells

Bio-Trac 43: iPSC I

Bio-Trac CRM 47: iPSC II: Induced Human Pluripotent Stem Cells (ihPSC); Differentiation to Neural Lineages

Bio-Trac CRM 49: iPSC: Making iPSC from Blood

Three Day Course
TRAC 48
July 29 - 31, 2013
Monday - Wednesday
REGISTER
TIME

9:00 - 5:00 p.m.

21 lecture/lab contact hours
FEE
Early Registration: $895 (by 6/1/13); $995
(Lecture & Hands-on Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences