TRAC 46: Genome Analysis and Mutation Detection
This intensive practical course is dedicated to genome analysis and mutation detection. It covers genotyping, RNA and gDNA quality control using a native capillary electrophoresis system as well as mutation analysis, methylation studies and microbial identification by pyrosequencing.
This is a hands-on course for all who wish to learn molecular biology techniques in DNA and RNA analysis. It is supported by experts and offers an access to automated systems. For the practical, a choice of samples will be prepared, but participants are also encouraged to provide their own samples for testing. The course will be comprised of lectures and hand-on sessions. Lectures will cover background knowledge pertaining to different applications, preparation of Standard Operating Procedures (SOPs) and analysis of the data.
Topics: Overview; Introduction QIAxcel CE system; Purification of gDNA using QIAcube automated system; QC of gDNA; Genotyping: Multiplex PCR, Singleplex PCR, Rep PCR: REP-, BOX-, ERIC-PCR, RAPID, VNTR, STR, RFLP; BioNumerics Plug in Software: Preparation of Dendrograms; NGS Library QC Using QIAxcel; SNP Confirmation or Biomarker Verification (GWAS & NGS data); Introduction Pyrosequencing; Pyro Applications for Mutation Analysis and Genotyping; QIAGEN Strategies for Methylation Analysis (Epitect and Pyro); Methylation Studies; Microbial ID (Identification and Subtyping)