Biotechnology Training Courses at the National Institutes of Health
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Building 10
Room 1N241 -
MSC 1115
Bethesda, MD



TRAC 43: iPSC I: Principles and Methods

The importance of induced pluripotent stem (iPS) cells in disease therapy and developmental biology is rapidly emerging and prompts this new 3-day lecture-lab course.

The emphasis of the course is on practical information that will help the researcher bring iPS technology to the laboratory. The staff conducting the course includes experts from academia and industry.

Lectures will discuss the expression of genes required for inducing pluripotency, methods of making (virus, RNA, plasmid) and maintaining iPS cells, growth conditions for differentiation (emphasis on hematopoietic and cardiac), and contrasts with embryonic stem cells.

Labs will cover techniques for staining for alkaline phosphatase and immunofluorescent markers of pluripotency on live cells. Furthermore, methods for picking iPS colonies and DNA transfections will also be included. Although the techniques will be conducted mostly on mouse cells, their applicability to human cells will be discussed.

Related Bio-Trac Courses:
Bio-Trac 18: Stem Cells

Bio-Trac 47: Human Pluripotent Stem Cells (hiiPSC); Differentiation Neural Lineages

Bio-Trac CRM 49: Making iPSCs from Blood

Bio-Trac CRM 54: Making Cardiomyocytes from iPSCs

Bio-Trac CRM 55: Engineering with CRISPR, TALENs, and ZFNs

TRAC 43-Fl
This workshop is only available on a contract basis.

See Trac CRM 47 for hiPSC

9:00 - 5:00 p.m.

21 lecture/lab contact hours
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences