TRAC 4: Cellular Immunology: Principles and Methods

The objective of this course, through lecture and laboratory sessions, is to learn those research approaches which form the foundation of our understanding of the immune system, with particular emphasis on the cellular elements and their roles in the orchestration of the immune response. Because this field is contributing to novel therapies and is in a high state of flux, due attention will be given to new directions.

Topics: Cells of the adaptive immune system and of the innate immune system; special focus on B lymphocytes and T lymphocytes; Interactions between antibodies and antigens; Genes encoding antibodies; Immunological memory and the mechanisms involved in recall responses; The Major Histocompatibility complex (MHC) and how it controls adaptive immune response;  Immunotolerance and how the immune system is kept under control; Immunotherapy; How to generate and produce monoclonal antibodies; Using a flow cytometer for the analysis of cells of the immune system; Immunological assays (T cell proliferation, cytotoxic T cell assays, Western Blotting/Immunostaining, ELISA)

Lecture:
9:00 - 4:00 p.m.
Monday
8:30 - 11:30 a.m.
Tuesday-Friday

Laboratory:
12:30 - 5:30 p.m.
Tuesday-Friday