Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 10
Room 1N241 -
MSC 1115
Bethesda, MD
20892-1115

301-496-8290

 

TRAC 37: Real Time, Quantitative and Digital PCR

Conventional PCR has revolutionized the detection and analysis of nucleic acids.  However, one of its major limitations has been the inability to accurately quantitate the amount of product, which reflects the amount of starting material, due to differing plateau effects among multiple samples. The need to accurately determine quantitative changes in gene expression has led to the adoption of real-time RT-PCR as the method of choice not only for quantitative gene expression but also for validating results obtained from array analyses and other techniques that evaluate gene expression changes on a global scale. This lecture/laboratory course is intended for those who have a fundamental background in PCR and will address the basic chemistries of real time PCR and the many platforms available.

Lecture and Laboratory Topics Include: Introduction to and Brief History of Real-Time PCR; Sample Processing: DNA/RNA Extraction and Purification for Real-Time PCR; Probe Chemistries and Real-Time Instrumentation including Amplification using Three Different Platforms; Assay Development; Quantitative Real-Time PCR; Multiplex Real-Time PCR; Array qPCR; Droplet Digital PCR (ddPCR).

Related Bio-Trac Course: Bio-Trac 26: PCR Methodology: Principles, Optimization and Applications

FIVE DAY COURSE
TRAC 37-SU
July 14 - 18, 2014
Monday - Friday
REGISTER-SU
TRAC 37-WN
Jan. 26 - 30, 2015
Monday - Friday
REGISTER-WN
TIME

9:00 - 5:00 p.m.

35 lecture/lab contact hours
FEE
$850 (Lecture & Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences