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TRAC 30: Small Interfering RNA (siRNA) & Functional Genomics
Studies of post-transcriptional gene silencing (PTGS) led to the discovery of the phenomenon of RNA interference (RNAi) and the role-played in that process by small interfering RNA (siRNA). Although only recently identified, siRNA molecules already are taking the research world by storm as their potential as functional genomic tools is beginning to be appreciated. Compared to antisense and knockout techniques, siRNA can more rapidly and effectively create loss-of-function phenotypes and they may even provide an approach for gene therapy. In this hands-on lecture-lab workshop participants will learn the latest information about RNAi and the use of siRNA as a functional genomics tool.
Lectures: Overview Antisense, Gene Knockouts, and siRNA; Gene Silencing and siRNAs; Delivery of siRNA to Eukaryotic Cells; DICER/RISC and the Mechanism of Action of RNAi; Chromosome Remodeling with Small RNAs; Development and siRNAs; RNAi and Epigenetics; siRNA and Cancer; RNAi and Drug Discovery; Design of siRNAs.
Laboratory: Comparison of Chemically Synthesized and in vitro Transcribed siRNAs to Induce Gene Silencing; Inducing Gene Silencing using siRNA Expression Plasmids Under Transient Selection; Silencing Gene Expression by PCR Based siRNA Expression Cassettes; Preparing siRNA Cocktails
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