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TRAC 3: Recombinant DNA Methodology II Use of Eukaryotes and Specialized Methods
This lecture and laboratory course complements TRAC 2 and is intended for those who are familiar with basic methods of recombinant DNA using plasmids and prokaryotic cells. This course will build on that information and focus on the use of eukaryotic recombinant DNA systems including "newer" vectors, selected host systems, and specialized techniques and applications which stem from opportunities created by recombinant DNA.
Topics: Construction of cDNA Libraries in Lambda Phage Vectors; Special Vectors and Hybrid Vectors; Synthesis of Gene Probes; Yeast: Cloning Systems and Host of Foreign DNA; Transient and Stable Expression of Recombinant DNA in Mammalian Cells; Viral Expression Vectors; Baculovirus System for Protein Synthesis; PCR - Methods and Applications; Eukaryotic Gene Expression: Analysis of Control Mechanisms; Nucleic Acid Probe Technology; Mapping and Characterization of Complex Genomes; Nucleic Acid Sequencing; Applications of Antisense DNA; Germ-line Transformation: Transgenic Animals; Current and Future Applications of Recombinant DNA; Screening of Lambda Phage Using Oligonucleotide Probes; In Vitro Mutagenesis; Transient Transfection Assays.
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