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TRAC 29: Laser Capture Microdissection: Methods for Microgenomic Analysis
Laser Microdissection systems allows for the procurement of homogeneous populations of cells from tissue and cytology samples containing heterogeneous populations of cells. The specificity of analyses are therefore much more representative of the disease process being studied. This approach to microdissection ensures that biological molecules, such as RNA and DNA, remain undamaged during the microdissection process. Downstream molecular analysis of these molecules produces accurate and assured results that have led to over 800 peer-reviewed publications by independent researchers.
In this four-day training program, participants learn to prepare and stain histological specimens for microdissection, then select and acquire homogenous cell populations using the Leica LMD, Arcturus XT, Veritas and PALM microdissection systems. Instruction emphasizes operation of these LCM Systems, appropriate tissue handling and sample preparation for subsequent DNA, RNA or protein analysis, and methods for proper molecular extraction. Lecture and detailed instructions to prepare samples for microarray hybridization are presented.
Topics: Overview of Laser Microdissection Technology (History, Theory, Applications); Practice on Arcturus, PALM, and Leica platforms; Means of Sample Preparation; Means of Slide Preparation; Staining Techniques; DNA/RNA/Protein Extraction Techniques; RT-PCR Versus Linear Amplification; Techniques to Measure the Quantity and Analyze the Quality of Isolated RNA/Protein; Microdissected Samples Using Linear Amplification; Labeling Amplified Materials to be Used on Various Microarray Platforms; Microarray Hybridzation Application and Use of Various Platforms; Mass Spec Applications
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