TRAC 28: Analytical Techniques for the Quantitation of Angiogenesis and Lymphoangiogenesis
The growth of blood vessels, a process known as angiogenesis, is essential in normal physiology and its imbalance contributes to numerous disorders including cancer and age-related macular degeneration. Antiangiogenic compounds are regarded as a very promising therapeutic tools for treatment of cancer and several other disorders.
This four day training course focuses on analytical techniques used to assess the in vitro/in vivo growth of blood vessel and lymphatic endothelial cells.
The course will be structured to have a combination of wet labs and lectures covering this subject matter. Hands-on operational training of the following techniques will be covered: 1) Chorioallantoic Membrane Assay (CAM), 2) Rat Aortic Ring Assay, 3) Directed In Vivo Angiogenesis Assay (DIVAA), 4) Endothelial Cell Migration Assay and 5) Endothelial Cell Tube Formation Assay.
Angiogenesis and it's Regulation by Redox Signaling; Angiogenesis and Tumor Endothelial Markers (TEM); Therapies Targeting Tumor Angiogenesis; Imaginng of Lymphangiogenesis; ECIS - A Morphological Biosensor for Cell Research; DIVVA: A Novel Method for Measuring Angiogenesis
Rat Aortic Ring Assay; Chick embryo chorioallantoic membrane (CAM) Assay; Tube Formation Assay; Growth Assay with Fluorescent Cells; Adhesion, Migration (ECIS) Tube Formation Assay; DIVVA CAM Assay