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TRAC 28: Analytical Techniques for the Quantitation of Angiogenesis and Lymphoangiogenesis
The growth of blood vessels, a process known as angiogenesis, is essential in normal physiology and its imbalance contributes to numerous disorders including cancer and age-related macula degeneration. Antiangiogenic compounds are regarded as a very promising therapeutic tools for treatment of cancer and several other disorders.
The NCI Angiogenesis Core Facility and the FAES is co-sponsoring a hands-on four day training course on analytical techniques used to assess the in vitro/in vivo growth of blood vessel and lymphatic endothelial cells.
The course will be structured to have a combination of wet labs and lectures covering this subject matter. Hands-on operational training of the following techniques will be covered: 1) Chorioallantoic Membrane Assay (CAM), 2) Rat Aortic Ring Assay, 3) Directed In Vivo Angiogenesis Assay (DIVAA), 4) Endothelial Cell Migration Assay and 5) Endothelial Cell Tube Formation Assay.
Lecture Titles:
Angiogenesis and it's Regulation by Redox Signaling; Angiogenesis and Tumor Endothelial Markers (TEM); Therapies Targeting Tumor Angiogenesis; Imaginng of Lymphangiogenesis; ECIS - A Morphological Biosensor for Cell Research; DIVVA: A Novel Method for Measuring Angiogenesis
Laboratory Topics:
Rat Aortic Ring Assay; Chick embryo chorioallantoic membrane (CAM) Assay; Tube Formation Assay; Growth Assay with Fluorescent Cells; Adhesion, Migration (ECIS) Tube Formation Assay; ; DIVVA CAM Assay
Course Director - Dr. Enrique Zudaire - NCI Angiogenesis Core Facility - zudairee@mail.nih.gov
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