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TRAC 27: Cryobiology: Principles and Methods
This lecture and laboratory course will provide an introduction to proteomics technology. Both principles and advanced methodologies will be discussed with an emphasis on protein identification tools, shotgun sequencing and bioinformatics technologies.
Topics: Introduction to Cryobiology: Biophysical and Biochemical Principles Involved in Cryobiology; Ice Formation; Seeding Temperature; Freezing Curves; Introduction to Gamete Freezing Methods; To Freeze or Not to Freeze; Equipment Description: Description of Different Cryogenic Vessels and Freezers, Vials and Straws, Inventory Systems; Embryo Slow Freezing Method; Two Step Freezing and Vitrification Methods; Introduction to Rapid, Ultra-Rapid and Vitrification Methods; Sperm Freezing; Introduction to Mouse Sperm Freezing and IVF; Ovary Freezing: Introduction to Ovary Freezing and Surgical Implantation.
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