TRAC 26: PCR Methodology: Principles, Optimization and Applications
From its conception in 1983 to its modern day use in a myriad of clinical and research applications, the Polymerase Chain Reaction (PCR) has revolutionized modern molecular biology. This lecture and laboratory course will focus on the Polymerase Chain Reaction and its applications in basic molecular biology research, genetics, molecular pathology, including cancer and genetic diseases and identification of viral, bacterial and other pathogens.
Topics: Topics include Basic PCR, Dissection of the PCR Cycle, Primer Design and Usage including Tools for Primer Design, Thermostable DNA Polymerases, Optimization, PCR Additives, Contamination Control, Reverse Transcription PCR (RT-PCR) PCR Mutagenesis, Cycle Sequencing, 5' and 3' RACE PCR, Multiplex PCR, Clinical and Forensic Applications, Practical Modifications to PCR.
Related Bio-Trac Course: Bio-Trac 37: Real Time and Quantitative PCR