TRAC 25: Proteomics: Principles and Methods

This lecture and laboratory course will provide an introduction to proteomics technology. Both principles and advanced methodologies will be discussed with an emphasis on protein identification tools, shotgun sequencing and bioinformatics technologies.

Topics: The power of 2D electrophoresis: its advantages, applications and data analysis tools for the examination of the spots of interest; high-throughput mass spectrometric-based protein identification: spot excision, in situ proteolysis, extraction of the cleavage products from the gel matrix, peptide purification and concentration as well as mass spectrometric sample preparation and analysis; isotope-coded affinity tag methods to identify and quantify low-abundance and membrane proteins; multidimensional high performance liquid chromatography - capillary electrophoresis separation of a protein digest prior to mass spectrometry for identifying low abundance proteins from complex biological mixtures; protein arrays for gene expression and molecular interaction screening; novel algorithms for protein sequence alignment to generate protein identities from partial or complete databases; new systems for obtaining detailed functional information and large-scale classification of proteins; homology modeling for predicting the 3D details of biomolecules of unknown structure.

9:00 - 5:00 p.m.