Biotechnology Training Courses at the National Institutes of Health
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Building 10
Room 1N241 -
MSC 1115
Bethesda, MD



TRAC 24: Principles and Applications of DNA Microarrays

Global transcription patterns as well as nucleotide polymorphisms can now be readily evaluated using DNA microarrays. This functional genomics technology offers a powerful, non biased approach to the analysis of molecular mechanisms associated with any type of physiological change in a cell. Moreover, DNA microarrays have become a prominent technological tool for genotype characterization. This lecture and laboratory course will show how DNA microarrays can be used for different applications in the biomedical sciences and will introduce hands-on training to a complex yet exciting technology.

Lecture Topics: Overview of DNA microarrays; Transcriptional analysis from single genes to whole genomes; Types and applications of microarrays; Use of microarrays for genotyping; comparative genome hybridization providing a molecular karyotype of the genome; Fabrication of DNA microarrays; microarray data analysis; Concepts of microarray bioinformatics; Dove-tailing expression data to genome sequences and other types of functional genomics; genotyping and resequencing using genechip technology; high throughput profiling platforms: development of tools for diagnosis and management of disease; introduction of new technologies – microfluidics-generated microarrays with in situ synthesized probes, Illumina bead technology.

Laboratory Topics: Isolation of total RNA; purification and quantitation of mRNA; fluorescent labeling of cDNA; Hybridization of cDNA to DNA arrays; Scanning and data collection; Analysis and display of data using clustering programs; Validation of microarray expression analysis by real-time and conventional RT-PCR.

Related Bio-Trac Course:  Bio-Trac 34: Advance Microarray Analysis and Pathway Integration

Sept. 15-19, 2014
Monday - Friday

9:00 - 5:00 p.m.

35 lecture/lab contact hours
$850 (Lecture & Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences