Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 10
Room 1N241 -
MSC 1115
Bethesda, MD
20892-1115

301-496-8290

 

TRAC 23: Flow Cytometry: Principles and Methods

This four day lecture/laboratory workshop will cover various applications of flow cytometry in basic research. Selected lecture topics will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; data analysis and presentation; technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis.

Lecture and Laboratory Topics: Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; Blocking with Antibodies); Software; Laser Scanning Cytometry; Proliferation using Bio-Tracers

FOUR DAY COURSE
TRAC 23-FL
Nov. 11-14, 2014
Tuesday - Friday
REGISTER-FL
TRAC 23-WN
Feb. 10-13, 2015
Tuesday - Friday
REGISTER-WN
TRAC 23-SP
May 5-8, 2015
Tuesday - Friday
REGISTER-SP
Time
9:00 - 5:00 p.m.
FEE
28 lecture/lab contact hours
$795 (Lecture & Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences