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TRAC 21: Mitochondrial Molecular Biology and Pathology Workshop
The purpose of this three day workshop is to provide a foundation of knowledge to those beginning to investigate mitochondrial function and biogenesis or those simply interested in understanding these essential subcellular organelles. Participants will learn of the important metabolic reactions occurring within the mitochondria and how nuclear and mitochondrial DNA lesions can result in a wide variety of mitochondrial disorders. The workshop will include a combination of lectures and hands-on laboratory experiments designed to familiarize the participants with the skills necessary to work with these organelles.
Topics: Structure and Function of Mitochondria; Replication of Mitochondrial DNA and Mitochondrial Genetics; Transcription and Processing of Mitochondrial RNA; Mitochondrial Protein Synthesis and Import; Molecular Methods for Working with Mitochondria; Mitochondrial Disorders: Molecular Lesions, Phenotypes, Mutation Detection and Approaches to Therapy. Isolation of mammalian mitochondria; Marker enzyme assays; Isolation of mtDNA and RNA; PCR amplification of mtDNA from total cellular DNA; Mutation detection by PCR/RFLP.
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