TRAC 20: Immunocytochemistry

Immunocytochemistry is an important research tool which has significant applications in topics as important and diverse as basic cell biology, carcinogenesis, apoptosis, genomics, clinical diagnosis, and intracellular migration and localization of molecules. This 3 day lecture and lab course will introduce the participants to the key methods and applications of immunocytochemistry and their underlying principles.

Topics: Introduction to Immunofluorescence; Conjugation of Proteins with Flurochromes; Tissue Preparation for Immunocytochemistry; Immunocytochemistry on Whole Mount Preparations; In situ Hybridization with Immunoprobes; Labeling and Flow Analysis of Cells; Applications of Flow Cytometry and Confocal Microscopy. Preparation of and Fluorescent Labeling of Cultured Cells; ABC-Peroxidase and Acid Phophatase Labeling of Sectioned Material; ELISA on Fixed Cells; Fluorescein Labeling of Nerve Cells in Whole Mount.

9:00 - 5:00 p.m.