Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 10
Room 1N241 -
MSC 1115
Bethesda, MD
20892-1115

301-496-8290

 

TRAC 20: Immunocytochemistry

Immunocytochemistry is an important research tool which has significant applications in topics as important and diverse as basic cell biology, carcinogenesis, apoptosis, genomics, clinical diagnosis, and intracellular migration and localization of molecules. This 3 day lecture and lab course will introduce the participants to the key methods and applications of immunocytochemistry and their underlying principles.

Topics: Introduction to Immunofluorescence; Conjugation of Proteins with Fluorochromes; Tissue Preparation for Immunocytochemistry; Immunocytochemistry on Whole Mount Preparations; In situ Hybridization with Immunoprobes; Labeling and Flow Analysis of Cells; Applications of Flow Cytometry and Confocal Microscopy. Preparation of and Fluorescent Labeling of Cultured Cells; ABC-Peroxidase and Acid Phophatase Labeling of Sectioned Material; ELISA on Fixed Cells; Fluorescein Labeling of Nerve Cells in Whole Mount.

THREE DAY COURSE
TRAC 20
New Date
July 28-30, 2014
Wednesday - Friday
REGISTER
TIME

9:00 - 5:00 p.m.

21 lecture/lab contact hours
FEE
$750 (Lecture & Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences