TRAC 2: Recombinant DNA Methodology I

This lecture and laboratory course is designed to provide the novice with an introduction to recombinant DNA technology. An approach emphasizing both principles and methodology provides the scientist with the essential fundamentals needed for gene cloning and for an appreciation of the strategies of recombinant DNA technology.

Topics: The Power of Recombinant DNA Technology-its advantages and applications; Recombinant DNA Safety Guidelines; Vector-Host Systems; Propagation and Maintenance of Bacterial Strains and Viruses; Isolation of Cloning Vector-bacteriophage and Plasmids; Construction of Genomic Libraries; Construction of cDNA Libraries; Extraction and Purification of mRNA from Eukaryotic Cells; Synthesis of cDNA; Screening for Differentially Expressed Genes; Microarrays; Differential Display; Nucleic Acid Sequencing and Site Directed Mutagenesis; Restriction Enzymes; Analysis of Nucleic Acids by Gel Electrophoresis; Insertion of the cDNA with Vectors; Transfection of Bacteria with Recombinant DNA; Identification and Analysis of Recombinant DNA Clones; Vector Systems which allow for the Expression of the Cloned Fragments in the Bacterial Host; Special Expression Vector Systems: Strategies in Applying Recombinant DNA; Gene Therapy

Lecture:
8:30 - 11:30 a.m.
Monday-Thursday
9:00 - 4:00 p.m.
Friday

Laboratory:
12:30 - 5:30 p.m.
Monday-Thursday