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TRAC 17: Apoptosis: Principles and Methods for Study of Normal and Pathological Biological Activity.
This 3-day lecture/laboratory course will serve to provide attendees with an appreciation of the complex role of apoptosis (programmed cell death) in organ development and maintenance of tissue homeostasis, aging, pathophysiology of disease and therapy. The selected lecture themes will present diverse examples which summarize the multiple roles of apoptosis and the rapid research advancement of the field. The laboratory experiments will serve to familiarize the attendees with the major morphological, cytochemical and molecular approaches to the identification, characterization and quantitation of apoptotic cells.
Topics: Overview of Necrosis and Apoptosis; Apoptosis in Organ Development: The C. elegans paradigm; Apoptosis in Maintenance of Homeostasis in the Adult; Significance of Apoptosis in Aging and Disease Development; Prevention of Apoptosis; Survival Factors and the bcl-2 Family; p53-dependent-and p53-independent Apoptotic Pathways; Apoptosis Networks in Oncogenesis; Death Factors: Fas and Death Domains; Apoptosis in the Immune System: Death by ICE; Preparation of Cells for Apoptosis Assays; The Annexin V Connection for Quantitation of Apoptotic Cells; Detection of Apoptosis in situ: Use of Terminal Deoxynucleotidyl Transferase Reaction (TUNEL assay); Utilizing Frozen and Paraffin-Embedded Tissue Sections for the Identification of Apoptotic Cells; Induction and Inhibition of Apoptosis: Detection of Nucleosomal Fragmentation via DNA Laddering Effect: Agarose Gel Electrophoresis; ELISA-Based Cell Death Detection Assays: Analysis of Apoptotic and Necrotic Cells
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