Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 60
Room 237
1 Cloister Court
Bethesda, MD
20814-1460

301-496-8290

 

TRAC 16: Expression, Detection & Purification of Recombinant Proteins in Prokaryotic & Eukaryotic Cells

This 3 day lecture laboratory course will give the student a good theoretical background as well as practical information and laboratory experience in the design, expression, detection and purification of recombinant proteins from a variety of expression systems.

Topics: Introduction to Protein Purification Techniques; Expression of Proteins in Prokaryotic and Eukaryotic Cells: Requirements, Advantages and Disadvantages; Prokaryotic Expression Systems: Strains, Tags and Purification Systems; Expression in Insect Cells: Baculovirus and Drosophila Systems; Expression in Mammalian Cells: Detection and Purification (Immunohistochemistry, Reporter Gene Assays, GFP, Tags for Expression/Purification Systems); Generation of Antibodies to Recombinant Proteins: System considerations and Antibody Purification; Expression in Yeast Cells (Pichia).

THREE DAY COURSE
TRAC 16 -FL
Dec. 10-12, 2008
REGISTER -FL
TIME

9:00 - 5:00 p.m.

21 lecture/lab contact hours
FEE
$750 (Lecture & Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences