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TRAC 16: Expression, Detection & Purification of Recombinant Proteins in Prokaryotic & Eukaryotic Cells
This 3 day lecture laboratory course will give the student a good theoretical background as well as practical information and laboratory experience in the design, expression, detection and purification of recombinant proteins from a variety of expression systems.
Topics: Introduction to Protein Purification Techniques; Expression of Proteins in Prokaryotic and Eukaryotic Cells: Requirements, Advantages and Disadvantages; Prokaryotic Expression Systems: Strains, Tags and Purification Systems; Expression in Insect Cells: Baculovirus and Drosophila Systems; Expression in Mammalian Cells: Detection and Purification (Immunohistochemistry, Reporter Gene Assays, GFP, Tags for Expression/Purification Systems); Generation of Antibodies to Recombinant Proteins: System considerations and Antibody Purification; Expression in Yeast Cells (Pichia).
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