Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 10
Room 1N241 -
MSC 1115
Bethesda, MD
20892-1115

301-496-8290

 

TRAC 16: Expression of Recombinant Proteins: Overcoming Challenges & Optimization for Downstream Applications

This 3 day lecture laboratory course will give the student a good theoretical background and practical experience in expression and purification of recombinant proteins from a variety of expression systems. Furthermore, the course will address new methods to overcome traditional challenges in recombinant protein expression and purification. Finally, students will be introduced to methods to prepare their samples for downstream applications such as immunofluorescence studies.

Topics: Introduction to Protein Purification Techniques; Expression of Proteins in Prokaryotic and Eukaryotic Cells: Requirements, Advantages and Disadvantages; Prokaryotic Expression Systems: Strains, Tags and Purification Systems; Expression in Insect Cells: Baculovirus and Drosophila Systems; Expression in Mammalian Cells: Detection and Purification (Immunohistochemistry, Reporter Gene Assays, GFP, Tags for Expression/Purification Systems); Generation of Antibodies to Recombinant Proteins: System considerations and Antibody Purification; Expression in Yeast Cells (Pichia).

THREE DAY COURSE
TRAC 16 -FL
Nov. 12-14, 2014
Wednesday - Friday
REGISTER -FL
TIME

9:00 - 5:00 p.m.

21 lecture/lab contact hours
FEE
$750 (Lecture & Laboratory)
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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences