Biotechnology Training Courses at the National Institutes of Health
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BIO-TRAC

FAES/NIH
Building 10
Room 1N241 -
MSC 1115
Bethesda, MD
20892-1115

301-496-8290

 

TRAC 1: Biotechnology Techniques

This lecture and laboratory course will provide the theoretical basis and the hands-on experience needed for the understanding and execution of those laboratory procedures which are widely used in biotechnology, molecular genetics, immunology, and biochemistry. While the subject matter will be discussed in the context of broadly stated research objectives, the emphasis will be on the techniques rather than on specific applications and strategies. Thus, the participants will acquire skill in the use of "generic" methods, and through an understanding of such methods, improve their effectiveness as workers in a contemporary research laboratory.

Topics: Recombinant DNA, Separation, and Monoclonal Antibody Procedures: these will include Gel Electrophoresis (acrylamide and agarose); Column Chromatography; Immunocytochemistry; ELISA; Spectrophotometry (quantitation; enzyme kinetics); Nucleic Acid Purification and Molecular Weight Determinations; Cell Separation Methods; Protein Separation and Quantitation; Liquid Scintillation (double label) Counting; Autoradiography (cellular and gross); and Restriction Enzyme Mapping; Gene Expression; Oligonucleotide Synthesis.

FULL SEMESTER
TRAC 1
Sept. 22 - 26, 2014
Monday - Friday
REGISTER
TIME

Lecture:
9:00 - 4:00 p.m.
Monday
8:30 - 11:30 a.m.
Tuesday-Friday

Laboratory:
12:30 - 5:30 p.m.
Tuesday-Friday

18 lecture hours;
20 lab hours
FEE
$850 (Lecture & Laboratory)

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Biotechnology Training Courses at the National Institutes of Health
Sponsored by the Foundation for Advanced Education in the Sciences