TRAC 1: Biotechnology Techniques

This lecture and laboratory course will provide the theoretical basis and the hands-on experience needed for the understanding and execution of those laboratory procedures which are widely used in biotechnology, molecular genetics, immunology, and biochemistry. While the subject matter will be discussed in the context of broadly stated research objectives, the emphasis will be on the techniques rather than on specific applications and strategies. Thus, the participants will acquire skill in the use of "generic" methods, and through an understanding of such methods, improve their effectiveness as workers in a contemporary research laboratory.

Topics: Recombinant DNA, Separation, and Monoclonal Antibody Procedures: these will include Gel Electrophoresis (acrylamide and agarose); Column Chromatography; Immunocytochemistry; ELISA; Spectrophotometry (quantitation; enzyme kinetics); Nucleic Acid Purification and Molecular Weight Determinations; Cell Separation Methods; Protein Separation and Quantitation; Liquid Scintillation (double label) Counting; Autoradiography (cellular and gross); and Restriction Enzyme Mapping; Gene Expression; Oligonucleotide Synthesis.

Lecture:
9:00 - 4:00 p.m.
Monday
8:30 - 11:30 a.m.
Tuesday-Friday

Laboratory:
12:30 - 5:30 p.m.
Tuesday-Friday